B73 RefGen_v4cen - Manual Reassembly of the 106Mb of centromeric and pericentromeric sequence RefGen_v4 genome assembly.

Reassemblies of the centromeric regions of Zea mays B73 constructed from existing, and publicly available PacBio read data generated for the B73 RefGen_v4 sequencing project, presented in Jiao et al. 2017 (Bioproject PRJNA10769, Pac Bio SRA Accession SRX1472849). Gaps in the centromeres and pericentromeres of the RefGen_v4 assembly were filled with previously unincorporated RefGen_v4 contigs or reads using unique junctions and the RefGen_v2 BAC by BAC assembly (NCBI Assembly Accession GCA_000005005.4) to walk across gaps. Assembly was assisted by Junction Viewer annotation software (Wolfgruber et al. 2010) and final sequence assembly using Geneious 6.0 software.