Protein thermostability predictions for Arabidopsis, maize, and poplar created using prokaryote protein optimal temperatures to estimate site-specific temperature sensitivity. Pfam domains were identified from the representative proteome from each plant species and aligned to corresponding prokaryote Pfam domains. Known OGTs from the prokaryotic Pfam domains were used to create estimates of optimal temperature and protein stability for each plant protein. Plant protein stability estimates were compared across tissues and organelles.